Description
Overview
Ideal for excitation of nucleic acids with UV of blue light. Unlike EtBR, SmartGlow is non mutagenic and does not require special methods of disposal.
Features
• Replaces hazardous ethidium bromide (EtBr)
• Better sensitivity than EtBr (down to 0.1ng)
• Excitation of both UV and blue light
• Ships at ambient temperature (store at 4°C)
• Two types: PS (Pre-Stain) or LD (Loading Dye)
• Compatible with SmartBlue™ Transilluminator
Protocol for SmartGlow PS
Prepare 100ml of agarose or polyacrylamide solution.
Add 5ul of SmartGlow PS stain to the gel solution before pouring gels.
For enhanced results, add SmartGlow PS to the running buffer at a ratio of 2.5ul to 5ul per 100ml. Adding SmartGlow PS to the running buffer will result in increased sensitivity and better detection of small quantities of nucleic acid.
After electrophoresis is complete, view the gel using a UV or blue light illuminator
Protocol for SmartGlow LD
Prepare agarose for polyacrylamide solution.
Pour gels.
Mix SmartGlow LD with samples and DNA markers at a 1:5 (dye to sample) dilution.
After electrophoresis is complete, view the gel using a UV or blue light illuminator