Description
Product Features:
• Rapidly generates high quality qPCR-ready cDNA
• Provides unbiased, complete sequence representation
• Thermostable SurePlus RT with RNase inhibitor allows for reactions at high temperature
• Optimized for use across a wide dynamic range
• Two-tube format allows for easy reaction set up
Product Details:
The SurePlus cDNA Synthesis Kit provides a quick, accurate and reproducible way to obtain high quality cDNA with a complete sequence representation for 4pg to 2?g of total RNA or mRNA.
The centerpiece of the SurePlus cDNA Synthesis Kit is qPCR RT – a thermostable reverse transcriptase that allows reactions to be carried out at higher temperatures, thus eliminating the problems created by secondary structure in the RNA. A potent RNase inhibitor maintains integrity of the starting material. Unbiased representation and synthesis are ensured by a precise mix of anchored oligo (dT) and hexamer primers in the 5X buffer system. Buffer and enzyme are supplied in separate tubes to allow for negative control reactions. The SurePlus qPCR mix is the ideal partner for quantitative PCR of cDNA.
