Description
Background
Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and osteoclast differentiation factor (ODF), is a member of the TNF superfamily. RANKL also has a function in the immune system, where it is expressed by T helper cells and is thought to be involved in dendritic cell maturation. The murine TRANCE cDNA encodes a type II transmembrane protein of 316 amino acids with a predicted cytoplasmic domain of 48 amino acids and extracellular of 247 amino acids. Mouse and human TRANCE share 85% amino acid sequence identity. TRANCE is primarily expressed in T cells and T cell rich organs, such as thymus and lymph nodes.
Source: E coli-derived with an N-terminal Met
Predicted Molecular Mass: 46 kDa
Specifications
Activity: The ED50 for this effect is typically 0.5-2ng/ml
Endotoxin Lever: <0.01 EU/ug of the protein.
Purity: >95% by SDS-PAGE gel under reducing conditions and visualized by silver stain.
Preparation and Storage
Reconstitution: 100ug/ml in sterile PBS.
Shipping: Dry ice. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -80°C as supplied.
- 2 month, 2 to 8°C under sterile conditions after reconstitution.
- 1 year, -70°C under sterile conditions after reconstitutions.
